Fig. 3

Construction of the platform for expressing the nanobody-HRP fusion protein. a Schematic presentation of the commercial vector pCMV-N1-EGFP changed into the vector to insert the main genes encoding vHRP. b Amino acid sequences encoded by the instering gene into the pCMV-N1-EGFP vector. Amino acids in the brown, yellow, blue, red, and green show the IgG signal peptide, HA tag, NDV-Nb5, HRP, and His tag, respectively