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Table 2 Optimised amount of NDV-NP protein as the coating antigen and dilution of NDV-Nb5-HRP fusions in medium using the indirect ELISA

From: Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

Different amounts of NDV-NP proteins (ng) Different dilutions of NDV-Nb5-HRP fusions in the medium
10−1 10−2 10−3 10−4
10 0.3315 0.237 0.11 0.058
20 0.798 0.5115 0.185 0.0595
40 1.822 0.847 0.3705 0.0515
80 2.5565 2.188 1.0945 0.185
160 2.7785 2.4215 1.317 0.223
320 2.908 2.643 1.5805 0.3005
640 3.05 2.8035 1.846 0.3505
  1. The optimal amount of NDV-NP protein and dilution of NDV-Nb5-HRP were selected when the OD450nm value of the indirect ELISA was approximately 1.0