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Table 3 Optimised dilution of tested chicken sera for cELISA

From: Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

No. serum Sera type 1:10 1:20 1:40 1:80 1:160 1:320 1:640 1:1280
1 Positive 0.14 0.16 0.24 0.44 0.73 0.97 0.98 0.93
Negative 0.90 1.03 1.06 1.06 1.02 1.09 1.02 1.08
P/N 0.15 0.16 0.23 0.42 0.72 0.89 0.96 0.87
2 Positive 0.10 0.13 0.24 0.50 0.80 1.00 1.12 1.03
Negative 0.90 1.03 1.00 1.02 0.99 1.01 1.13 1.16
P/N 0.11 0.13 0.24 0.48 0.81 0.99 0.99 0.89
3 Positive 0.08 0.11 0.16 0.38 0.73 0.94 1.11 1.12
Negative 1.01 1.09 1.10 1.11 1.09 1.17 1.14 1.20
P/N 0.08 0.10 0.15 0.35 0.67 0.80 0.97 0.94
4 Positive 0.08 0.10 0.14 0.24 0.55 0.84 0.96 1.02
Negative 0.89 1.00 0.90 1.00 1.02 0.89 1.08 1.06
P/N 0.09 0.10 0.16 0.24 0.54 0.95 0.89 0.96
  1. Four positive and negative chicken sera were separately used for cELISA detection. The dilutions of sera were 1:10, 1:20, 1:40, 1:80, 1:160, 1:320, 1:640, and 1:1280. The best dilution was selected when the OD450nm value of positive to negative (P/N) sera was smallest