Times (min) of color reaction | Sera type | Incubation times (min) of chicken sera, NDV-Nb5-HRP fusions and antigens | |||||||
---|---|---|---|---|---|---|---|---|---|
5 | 10 | 15 | 20 | 25 | 30 | 45 | 60 | ||
10 | Positive | 0.69 | 0.86 | 0.52 | 0.54 | 0.48 | 0.24 | 0.24 | 0.29 |
Negative | 0.75 | 0.89 | 0.94 | 0.97 | 1.02 | 0.99 | 1.01 | 1.11 | |
P/N | 0.92 | 0.97 | 0.55 | 0.56 | 0.47 | 0.24 | 0.23 | 0.26 | |
15 | Positive | 0.70 | 0.63 | 0.50 | 0.47 | 0.39 | 0.25 | 0.26 | 0.29 |
Negative | 0.80 | 0.75 | 0.87 | 1.00 | 0.97 | 1.02 | 1.15 | 1.12 | |
P/N | 0.86 | 0.84 | 0.57 | 0.47 | 0.40 | 0.25 | 0.23 | 0.26 |