Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

Table 5 Comparisons of the developed cELISA with HI test and with commercial ELISA kit by detecting clinical chicken sera and egg yolks

Samples	Number	cELISA	Commercial ELISA kit		Agreement (%)	Kappa value	HI test		Agreement (%)	Kappa value
Samples	Number	cELISA	+	⁻	Agreement (%)	Kappa value	+	−	Agreement (%)	Kappa value
Chicken sera	360	+	359	1	97.83	0.793	360	0	98.10	0.742
Chicken sera	8	−	6	2	97.83		8	0	98.10
Egg yolks	96	+	96	0	100		96	0	100
Egg yolks	12	−	0	12	100		0	12	100

ISSN: 1477-3155