Fig. 4

Detection of V. parahaemolyticus (Vp) by BMP-A-Ab in comparison with commercial immunomagnetic bead/Ab complex (MB-A-Ab). a Detection of Vp using ∆F-BMP-FA (schematic). Step I: ∆F-BMP-FA is coupled to anti-Vp Ab to construct BMP-A-Ab. Step II: BMP-A-Ab captures Vp. Step III: capture by magnetic rack. Step IV: captured Vp is resuspended and spread on plate. b Comparison of Vp capture rates for BMP-A-Ab vs. MB-A-Ab with three concentrations of Vp. Capture rate was consistently greater for BMP-A-Ab. Maximal capture rate was 93%, for Vp number 630 ± 25 per mL