Fig. 2

Human dermal fibroblast (HDF) adhesion in vitro studies on polyurethane (PU), argon (Ar) and Medpor scaffolds. a HDF morphology after 24 h using F-actin staining on the PU and Medpor scaffolds. Scale bar refers to 30 μm. b Human Dermal Fibroblast (HDF) adhesion after 24 h on PU and Medpor scaffolds. P values * < 0.05 ** < 0.01. c RT-qPCR analysis of adhesion related expression markers after 24 h on PU and Medpor scaffolds. Ar showed significantly greater expression of adhesion markers than Medpor and PU (PU vs Ar p < 0.05, Medpor vs Ar *p < 0.01). Fold changes represents difference compared to housekeeping gene GAPDH of HDFs cell growing on tissue culture plastic. d HDF DNA content over 14 days on PU and Medpor scaffolds. Ar showed greater DNA content at 1, 4, 7, 10 and 14 days compared to PU and Medpor. PU; Unmodified polyurethane