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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Argon plasma modified nanocomposite polyurethane scaffolds provide an alternative strategy for cartilage tissue engineering

Fig. 4

Assessment of Extracellular Matrix (ECM) formation by the human dermal fibroblasts (HDFs) after 14 days on polyurethane (PU), argon (Ar) and Medpor scaffolds. a RT-qPCR Analysis of ECM formation on all scaffolds. Ar showed a significantly greater expression of Collagen I (Col I), Collagen III (Col III), elastin, fibronectin (Fn) and laminin (Lm) after 14 days than Medpor and PU (*p < 0.05). Fold change represents difference to housekeeping gene GAPDH of HDFs growing on tissue culture plastic. b Collagen Type I expression by immunocytochemistry of the HDFs after 14 days on all scaffolds. Green; Collagen Type I, Blue: Nuclei staining using DAPI. Scale bar refers to 50 μm. c Total collagen secretion by the HDFs on all scaffolds. d Hydroxyproline secretion by the HDFs on all scaffolds. P values * < 0.05 ** < 0.01 ***p < 0.001. PU; Unmodified polyurethane

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