Fig. 2
From: Nanocomposites as biomolecules delivery agents in nanomedicine
Enzyme immobilization on nanocarriers through covalent binding or physical absorption processes. EDC/NHS is a very common coupling method for creation of amide bonds. This coupling method uses chemical linkers that react with certain groups on the molecules. Therefore, the reaction is more specific and controllable than the passive absorption method, and the number of covalently conjugated ligands can be optimized to the particular application