Fig. 5

Distribution of Cy5.5-AuNPs in ICM-injected mice. Confocal images of sections (maximum projections) from the pons/medulla of mice sacrificed 30 days after ICM injections. Acquisitions were done to capture Cy5.5 and Au signals in control mice—i.e. receiving Cy5.5 (A, C, E and G)—and in mice receiving Cy5.5-AuNPs (B, D, F and H). A, B Triple labelling for NeuN, Cy5.5 and Au. Arrows indicate cells that are shown at high magnifications in insets. C, D Confocal images of adjacent sections labelled for Cy5.5, Au and GFAP. E, F Triple labelling for MBP, Cy5.5 and Au. Arrow in F indicates a region that is shown at high magnification in the inset. G, H Confocal images of sections labelled for Iba1, Cy5.5 and Au. The arrow in H indicates one Iba1⁺ cells that is shown at high magnification in the inset (n = 3 for each group). I Au nanoparticles localized in vacuoles of parenchymal cells of brains sampled at day 15. Arrows indicate several Au particles in a putative lysosome of a cell (n = 2). Scale bar 50 µm for A–H, 0.1 µm for I