Fig. 6

Distribution of Cy5.5-AuNPs⁺NeuN⁺ in ICM-injected mice. A, B FLI scans in a control mouse (Cy5.5) and in a mouse receiving Cy5.5-AuNPs both sacrificed 15 days after the injection. The Epifluorescence scale is plotted on the right side of A. Representative confocal scans for NeuN, Cy5.5 and Au in the pons/medulla of these mince are shown in C, D (n = 3 for each group). D Is a confocal image obtained stitching six independent pictures. Percentages of NeuN⁺Cy5.5⁺Au⁺ were established in mice receiving Cy5.5-AuNPs scoring cells in the superimposed ROI (200 × 500 µm) and data are plotted in E. Arrows of D show NeuN⁺Cy5.5⁺Au⁺ cells. F A confocal cross section of a NeuN⁺ cells expressing Au and Cy5.5. Scale bar 100 µm for C, D and 10 µm for F