Fig. 5

Analyses of tyrosine hydroxylase (TH) (a–c), dopamine active transporter (DAT) (d–e) and the high-affinity choline transporter 1 (CHT1) (f–h) were performed at day in vitro (DIV) 7 to evaluate the effect of SiPCL-NP-exposure for 24 h on DIV1 (a) and on DIV4 and DIV6 (b, c) on the phenotype of SH-SY5Y cells. Undifferentiated cells (Undiff) and cells differentiated with retinoic acid (RA) or staurosporine (ST) were investigated in parallel. Histograms depict the levels of TH, DAT and CHT1 in relation to the loading control (actin) given in arbitrary numbers. Representative Western blots are shown for DIV4 exposure above the respective histograms. Image J was used for the analysis. Error bars correspond to SEM. Significant differences between control- and NP-exposed-groups are labeled with asterisks (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001)