Fig. 6

Analyses of differentiation markers [phosphatidylinositol-3-kinase (PI3-K/Akt) and of mitogen-activated-protein-kinase/extracellular-signal-regulated-kinase (MAP-K/ERK)] were performed at day in vitro (DIV) 7 to evaluate the effect of SiPCL-NP-exposure for 24 h on DIV4 in SH-SY5Y cells. Undifferentiated cells (Undiff) cells and cells differentiated with retinoic acid (RA) or staurosporine (ST) were assessed in parallel. Histograms (a, b) depict the ratio of phosphorylated Akt (P-Akt) (a) and phosphorylated MAP-K (P-p42/44 MAP-K) (b) to total Akt and p42/44 MAP-K, respectively. Actin was used as a loading control. Representative Western blots are shown above the histograms. Image J was used for the analysis. Error bars correspond to SEM. Significant differences between control- and NP-exposed-groups are labeled with asterisks (*p ≤ 0.05)