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Fig. 8 | Journal of Nanobiotechnology

Fig. 8

From: Silica nanoparticle-exposure during neuronal differentiation modulates dopaminergic and cholinergic phenotypes in SH-SY5Y cells

Fig. 8

Analyses of the Wnt-pathway markers, β-catenin and glycogen synthase kinase-3 β (GSK-3β), were performed at day in vitro (DIV) 7 to evaluate the effect of SiPCL-NP-exposure for 24 h on DIV4 in SH-SY5Y cells. Undifferentiated cells (Undiff) and cells differentiated with retinoic acid (RA) or staurosporine (ST) were examined in parallel. Histograms depict β-catenin (A) and GSK-3β (E) levels in relation to the loading control (actin). Representative Western blots are shown above the histograms. Image J was used for the analysis. Error bars correspond to SEM. Representative immunofluorescence microscopy images of β-catenin-stained cells (green) are depicted in BD (B = Undiff NP DIV4, C = RA NP DIV4, D = ST NP DIV4) and GSK-3β (green) in FH (F = Undiff NP DIV4, G = RA NP DIV4, H = ST NP DIV4). NPs are shown in red and cell nuclei in blue. Magnification ×630, scale bar = 10 μm

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