Silica nanoparticle-exposure during neuronal differentiation modulates dopaminergic and cholinergic phenotypes in SH-SY5Y cells

Table 1 Primary antibodies

Primary antibody	Host	Dilution		Company
Primary antibody	Host	IF	WB	Company
phospho-Akt	Rabbit	–	1:1000	Cell signaling
Akt	Rabbit	–	1:1000	Cell signaling
GSK-3β	Rabbit	1:400	1:1000	Cell signaling
TH	Rabbit	1:500	1:1000	Cell signaling
β-catenin	Rabbit	1:400	1:500	Cell signaling
phospho-p44/42 MAP-K	Mouse	–	1:1000	Cell signaling
p44/42 MAP-K	Mouse	–	1:1000	Cell signaling
CHT1	Mouse	1:250	1:500	Santa Cruz
β-actin	Mouse	–	1:20,000	Sigma-Aldrich
MAP-2	Mouse	–	1:500	Sigma-Aldrich
β-3-tubulin	Mouse	1:500	–	Sigma-Aldrich
α/β-synuclein	Mouse	1:250	–	Santa Cruz
DAT	Rat	–	1:1000	Millipore

CHT1 choline transporter 1, DAT dopamine active transporter, GSK-3 β glycogen synthase kinase 3 β, IF immunofluorescence, MAP-2 microtubule associated protein 2, MAP-K mitogen activated protein kinase, TH tyrosine hydroxylase, WB western blot

ISSN: 1477-3155