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Table 1 Primary antibodies

From: Silica nanoparticle-exposure during neuronal differentiation modulates dopaminergic and cholinergic phenotypes in SH-SY5Y cells

Primary antibody Host Dilution Company
IF WB
phospho-Akt Rabbit 1:1000 Cell signaling
Akt Rabbit 1:1000 Cell signaling
GSK-3β Rabbit 1:400 1:1000 Cell signaling
TH Rabbit 1:500 1:1000 Cell signaling
β-catenin Rabbit 1:400 1:500 Cell signaling
phospho-p44/42 MAP-K Mouse 1:1000 Cell signaling
p44/42 MAP-K Mouse 1:1000 Cell signaling
CHT1 Mouse 1:250 1:500 Santa Cruz
β-actin Mouse 1:20,000 Sigma-Aldrich
MAP-2 Mouse 1:500 Sigma-Aldrich
β-3-tubulin Mouse 1:500 Sigma-Aldrich
α/β-synuclein Mouse 1:250 Santa Cruz
DAT Rat 1:1000 Millipore
  1. CHT1 choline transporter 1, DAT dopamine active transporter, GSK-3 β glycogen synthase kinase 3 β, IF immunofluorescence, MAP-2 microtubule associated protein 2, MAP-K mitogen activated protein kinase, TH tyrosine hydroxylase, WB western blot