Secondary antibody | Dilution | Use with corresponding primary for detection of | Company | |
---|---|---|---|---|
IF | WB | |||
dk anti-rabbit IgG HRP | – | 1:5000 | phospho-Akt; Akt; TH | Novex |
– | 1:10,000 | GSK3-b; β-catenin |  | |
dk anti-rabbit IgG AF 488 or 647 nm | 1:200 | – | GSK3-b; β-catenin; TH | Invitrogen |
dk anti-mouse IgG HRP | – | 1:5000 | phospho-p44/42 MAP-K; p44/42 MAP-K; MAP-2 | Novex |
– | 1:50,000 | β-actin |  | |
dk anti-mouse IgG AF 488 nm | 1:200 | – | CHT1; β-3-tubulin; α/β-synuclein | Invitrogen |
dk anti-rat IgG HRP | – | 1:2000 | DAT | Novex |