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Table 2 Antibodies

From: Silica nanoparticle-exposure during neuronal differentiation modulates dopaminergic and cholinergic phenotypes in SH-SY5Y cells

Secondary antibody

Dilution

Use with corresponding primary for detection of

Company

IF

WB

dk anti-rabbit IgG HRP

–

1:5000

phospho-Akt; Akt; TH

Novex

–

1:10,000

GSK3-b; β-catenin

 

dk anti-rabbit IgG

AF 488 or 647 nm

1:200

–

GSK3-b; β-catenin; TH

Invitrogen

dk anti-mouse IgG HRP

–

1:5000

phospho-p44/42 MAP-K; p44/42 MAP-K; MAP-2

Novex

–

1:50,000

β-actin

 

dk anti-mouse IgG

AF 488 nm

1:200

–

CHT1; β-3-tubulin; α/β-synuclein

Invitrogen

dk anti-rat IgG HRP

–

1:2000

DAT

Novex

  1. AF Alexa Fluor, dk donkey, HRP horseradish peroxidase, IF immunofluorescence, WB Western blot