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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Mild thermotherapy and hyperbaric oxygen enhance sensitivity of TMZ/PSi nanoparticles via decreasing the stemness in glioma

Fig. 4

mRNA and protein analysis in NCH-421K cells after different treatments. a–d mRNA changes of SOX2, Nestin, HIF-1α and VEGF in NCH-421K cells after different treatments. NCH-421K cells were treated with 400 μM TMZ for 72 h (n = 3; *P < 0.05, **P < 0.01, ***P < 0.001). e Western blot analysis of SOX2, Nestin, HIF-1α and VEGF in NCH-421K cells after different treatments. NCH-421K cells were treated with 400 μM TMZ for 24 h. f–i The relative protein content of SOX2, Nestin, HIF-1α and VEGF in NCH-421K cells after different treatments (n = 3; *P < 0.05, **P < 0.01, ***P < 0.001)

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