Fig. 5

Effects of TMZ/PSi combined with PTT and HBO on C6 cells after different treatments. a–d mRNA changes of SOX2, Nestin, HIF-1α and VEGF in C6 cells after different treatments (n = 3; *P < 0.05, **P < 0.01, ***P < 0.001). C6 cells were treated with 400 μM TMZ for 72 h. e Western blot analysis of SOX2, Nestin, HIF-1α and VEGF in C6 cells after different treatments. C6 cells were treated with 400 μM TMZ for 24 h. f–i The relative protein content of SOX2, Nestin, HIF-1α and VEGF in C6 cells after different treatments (n = 3; *P < 0.05, **P < 0.01, ***P < 0.001)