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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: G2-S16 dendrimer microbicide does not interfere with the vaginal immune system

Fig. 4

Quantification of gene expression on treated iDCs M-MΦs and GM-MΦs. TLR1–10 mRNA expression was determined by RT-qPCR in ac M-macrophages (M-MΦs), df GM-macrophages (GM-MΦs) or gi immature dendritic cells (iDCs) treated with G2-S16 (1 µM for iDCs and 10 µM for M- and GM-MΦs) for a, d, g 3 h, b, e, h 6 h and c, f, i 18 h. Insufficient detection of certain TLR mRNA expression levels by RT-PCR was not included here to simplify the representation. Data shows relative mRNA levels; analyzed gene was normalized to TATA box binding protein expression (TBP) and referred to non-treated cells. Individual values (dots) and mean (bar) of at least three independent experiments are shown. Dashed lines indicate threshold values

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