Table 1 Real time PCR primer pairs used for quantification of human TLR mRNA
From: G2-S16 dendrimer microbicide does not interfere with the vaginal immune system
TLR | Forward primer | Reverse primer | Amplicon size (bp) |
|---|---|---|---|
LR1 | GGTCTTGCTGGTCTTAGGAGAGAC | CTGAAGTCCAGCTGACCCTGTAGCTTCACG | 372 |
TLR2 | GGCCAGCAAATTACCTGTGTG | CTGAGCCTCGTCCATGGGCCACTCC | 637 |
TLR3 | CGGGCCAGCTTTCAGGAACCTG | GGCATGAATTATATATGCTGC | 400 |
TLR4 | TGCAATGGATCAAGGACCAGAGGC | GTGCTGGGACACCACAACAATCACC | 449 |
TLR5 | CCTCATGACCATCCTCACAGTCAC | GGCTTCAAGGCACCAGCCATCTC | 355 |
TLR6 | CCAAGTGAACATATCAGTTAATACTTTAGGGTGC | CTCAGAAAACACGGTGTACAAAGCTG | 358 |
TLR7 | CTCCCTGGATCTGTACACCTGTGAG | CTCCCACAGAGCCTTTTCCGGAGCT | 551 |
TLR8 | GTCCTGGGGATCAAAGAGGGAAGAG | CTCTTACAGATCCGCTGCCGTAGCC | 581 |
TLR9 | GCGAGATGAGGATGCCCTGCCCTACG | TTCGGCCGTGGGTCCCTGGCAGAAG | 510 |
TLR10 | CAGAGGTCATGATGGTTGGATGG | GACCTAGCATCCTGAGATACCAGGGCAG | 256 |