Fig. 5

Confocal microscope images of reactivation and LIVE/DEAD cells. J89GFP or THP89GFP cell lines were treated with BRY (100 nM), G2-S16 (10 µM) or G3-S16 (1 µM) for 48 h. After incubation, cells were stained with NUCLEAR-ID^® Blue/Red. Blue: live cells. Green: HIV-1 reactivation. Red: cellular death. Non-treated (NT) cells were used as cell viability control. DMSO was used as cell death control. NT non-treated control; DMSO dimethyl sulfoxide; BRY bryostatin. Representative images of two independent experiments performed in duplicate are shown