Fig. 2

The inflammatory cue changes slightly the proteome of CardAP-EVs. Peptides were derived from unstimulated EVs (unstimulated) and cytokine stimulated EVs (cytokine stimulated) by an overnight digestion with trypsin. By liquid chromatography/electron spray ionization mass spectrometry (LC/ES MS) obtained mass spectra were evaluated by MASCOT software searching for protein matches in the SwissProt 51.9 database. a The interaction of proteins was visualized with the help of String database. Protein interactions were shown as connecting lines and were categorized as known interactions (grey connecting lines). The detected proteins are shown as nodes, which appear coloured due to their biological process or localisation (extracellular exosome = red, positive regulation of cellular process = green, angiogenesis = yellow, wound healing = blue, regulation of immune system process = magenta). b Shown is a heat map of the exponentially modified protein abundance index (emPAI) for 35 selected proteins from in total 186 detected proteins and for both EV variants from three CardAP donors. Not detected proteins corresponds to an emPAI value of 0