Fig. 6

The toxicity of afatinib in various formulations on normal cells. Cytotoxic effect of various formulations on rat intestinal IEC-6 cells or hemolysis effect of these formulations on red blood cells (RBCs). IEC-6 cells were treated with a different concentrations of afatinib and b afatinib in various LPNs for 24 h, 48 h or 72 h. Cell viability was measured by SRB assay. CTR: cell control; *P < 0.05: compared with Afa for 24 h; ^†P < 0.05: compared with Afa for 48 h; ^‡P < 0.05: compared with Afa for 72 h by Student’s t-test. c Hemolysis effect was determined by incubating afatinib in various formulations with RBCs at 37 °C for 24 h. Triton-X was used as a positive control. Lipofectamine 3000 (Lipo 3000) is a commercial transfection reagent. Values are the mean ± SD (n = 3)