Fig. 7

Cytotoxic effect of afatinib in various formulations on Caco-2 cells. Caco-2 cells were treated with a different concentration of afatinib and b 10 nM of afatinib in various LPNs at pH 7.4 or 6.5 for 72 h. Cell viability was measured by SRB assay. Values are the mean ± SD (n = 3). *P < 0.05: compared with CTR at pH 7.4, ^†P < 0.05: compared with Afa at pH 7.4, ^‡P < 0.05: compared with Afa/LPN at pH 7.4, ^¶P < 0.05: compared with Afa/LPN-H at pH 7.4, ^§P < 0.05: compared with Afa/LPN at pH 6.5, ^ǂ< 0.05: compared with Afa/LPN-H at pH 6.5 by Student’s t-test