Fig. 5

Adhesion and transmigration of APS-MNP-loaded OT-I CD8⁺ T cells across an endothelium. a Representative confocal microscopic images of OT-I CD8⁺ T cells, treated with APS-MNPs or not, migrating across a monolayer of murine endothelial cells (SVEC4-10 cell line). The upper panel represents the apical part of the monolayer, while the lower panel represents the basal part (OT-I CD8⁺ T cells (green), cytoskeleton (red)). Scale: 100 μm. Quantification of (b) adhesion and (c) transmigration of OT-I CD8⁺ T cells in the presence of increasing concentrations of APS-MNPs across murine endothelial cells. Mean values ± SD are shown for three different experiments, analysing at least 50 fields per condition, *p < 0.05, **p < 0.01, ***p < 0.001