Fig. 6

In vitro magnetic retention of APS-MNP-loaded OT-I CD8⁺ T cells and their chemotactic response. a Displacement of OT-I CD8⁺ T cells in the direction of the magnetic gradient (Y axis) after being treated with APS-MNPs or not and exposed to different EMFs. Cell displacement was quantified by analysing at least 100 cells per video using Imaris software. b Migratory capacity of OT-I CD8⁺ T cells after treatment with APS-MNPs in response to a specific chemotactic gradient and in the presence or absence of an EMF in the same direction. The results were normalised against a control well (in absence of a transwell assay). The results shown (mean ± SD) are representative of three or four independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001