Fig. 2

Biodistribution of CCOL2A1 gene in various tissues from vaccinated normal rats. pcDNA-CCOL2A1 was intramuscularly injected into normal Wistar rats with a single dose of 300 μg/kg (N = 6). On days 3, 7, 10, 14, 21, 28, 35 and 42 after vaccination, a panel of 10 tissues/organs were harvested and processed for qPCR determination. Data are expressed as PCN/μg RNA; LOD = 200 PCNs/μg RNA and LLOQ = 1000 PCNs/μg RNA. For graphing purposes, values from spleen, thymus, ovary, testis and blood are not shown which are less than the LOD or above the LOD but below the LLOQ. *P < 0.05 comparing values of various time-points after vaccination vs. the one of NC using the t-test. “NC” represent “normal control”