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Table 2 The amplified fragment size, primer and probe sequences of CCOL2A1 and β-actin by quantitative polymerase chain reaction

From: Dynamic profiles, biodistribution and integration evaluation after intramuscular/intravenous delivery of a novel therapeutic DNA vaccine encoding chicken type II collagen for rheumatoid arthritis in vaccinated normal rodent

Gene

Primer/probe name

Prime/probe sequence

Fragment size (bp)

Rat β-actin

Rat β-actin F-primer

5′-CTCATGCCATCCTGCGTCT-3′

116

Rat β-actin R-primer

5′-ACGCACGATTTCCCTCTCA-3′

Rat β-actin probe

5′-TGGCCGGGACCTGACAGACTACC-3′

CCOL2A1

CCOL2A1 F-primer

5′-TCTTGTTGGTCCCAGAGGTGA-3′

118

CCOL2A1 R-primer

5′-ACCCTTGGGTCCGTCAGTG-3′

CCOL2A1 probe

5′-CGTGGATTCCCCGGTGAACGC-3′