Fig. 6From: A novel biotinylated nanobody-based blocking ELISA for the rapid and sensitive clinical detection of porcine epidemic diarrhea virusSpecificity analysis of the blocking ELISA. A 96-well plate was coated with the PEDV N protein (100 ng/well) and then blocked. Dilute sera positive for 7 different pathogens (JEV, PPV, PRV-gE, PRV-gB, PCV2, PRRSV, TGEV and PEDV) were added to the wells. BiNb2 was then added to the wells, and HRP-conjugated streptavidin was added to the wells. Values are the mean PI (%) of three well replicatedBack to article page