Skip to main content

Table 1 The enrichment nanobodies in the VHH library were detected through three consecutive rounds of biopanning, and PEDV N specific nanobodies were enriched in the VHH library by approximately 1650 fold

From: A novel biotinylated nanobody-based blocking ELISA for the rapid and sensitive clinical detection of porcine epidemic diarrhea virus

Round of panning

Input phage (pfu/well)

P output (pfu/well)

N output (pfu/well)

Recovery (P/input)

P/N

1st round

4.4 × 1011

3.5 × 105

7.3 × 104

7.9 × 10−7

0.047

2nd round

2.0 × 1011

9.7 × 107

1.4 × 106

4.8 × 10−5

69

3rd round

5.2 × 1011

3.8 × 109

2.3 × 106

7.3 × 10−3

1.65 × 103