Fig. 3
From: Enhanced osteogenesis of quasi-three-dimensional hierarchical topography
In vitro evaluation of osteogenic differentiation of osteoblast on various substrates. a Western blot analysis of relative protein expression of early and middle-stage osteogenic differentiation markers ALP and COL-I, respectively, after 5, 7 and 14 days of culture. And b the corresponding PCR results of relative mRNA expression. c ELISA assay analysis of relative protein expression of later osteogenic differentiation marker osteocalcin (OC) after 14 days of culture. d SEM observation (right) and the corresponding EDS Ca elemental analysis (left) of calcium nodule after 21 days of culture. e Analysis of the relative calcium content