Fig. 5

Digital images and quantification of colony counts of P727 isolate in activated macrophages after treatment with negative control (cells were treated with culture medium), MXF and its nanoformulations at 37 °C for 4 and 24 h at 1/4 × MIC of MXF (4 μg/mL). After treatment, cells were harvested and lysed with Triton X-100 for 15 min, then cell suspensions were diluted 10-fold or 100-fold with saline and plated on agar plates at 37 °C for 24 h. The number of colonies was counted to assess the antibacterial effect of different formulations. **, statistically different at p < 0.01 vs control; ^##, statistically different at p < 0.01 vs MXF