Fig. 3
From: Isolation of microglia-derived extracellular vesicles: towards miRNA signatures and neuroprotection
UC-ODG isolation workflow. a Nanoparticle Tracking Analysis (NTA) of microglial EVs isolated with UC-ODG procedure from three independent samples. The number of particles/mL for each fraction is presented with a black dot in each replicate. The median value is represented by a green bar. b The total RNAs were extracted from fractions F4–F6 to perform Tailing+ vs. Tailing‒ RT-PCR experiments in order to suggest 21 putative miRNAs in microglial EVs. c From a novel microglial EV isolation with UC-ODG procedure, a RNase A digestion of EV-positive F4–F6 fractions was performed to identify 6 miRNAs in EVs. d The PCR products were sub-cloned and sequenced in order to validate the miRNA sequence. They represent the miRNA sequence (blue) and the universal reverse primer used in the experiment (red)