Fig. 5
From: Isolation of microglia-derived extracellular vesicles: towards miRNA signatures and neuroprotection
Influence of leech microglial EVs on neurite outgrowth. a Rat primary neurons were cultured with either 105, 106, 107 EVs (P2-EV+) or P3-EV‒ as control condition for 9 h, 24 h and 48 h. The measures of neurite length and number were overall made on cell population and showed a higher outgrowth under 106 and 107 EVs compared to control. b The images show neurons after T9h, T24h and T48h for each condition. Scale bars correspond to 20 µm. The significance was calculated by one-way ANOVA followed by Tukey post hoc test (**p < 0.01, ***p < 0.001, ****p < 0.0001, error bars: standard deviation)