Skip to main content
Fig. 7 | Journal of Nanobiotechnology

Fig. 7

From: Breaking the selectivity-uptake trade-off of photoimmunoconjugates with nanoliposomal irinotecan for synergistic multi-tier cancer targeting

Fig. 7

Multi-tier cancer targeting. a Schematic of multi-tier cancer targeting mechanism: (1) EGFR binding, endocytosis, and proteolysis of PIC–Nal–IRI, (2) PIT-mediated depolarization of mitochondrial membrane potential, and (3) irinotecan-induced DNA damage, leading to synergistic cell killing. b Immunoblotting of EGFR and γ-H2AX expression in OVCAR-5 cells at different time points after treatment. Quantitative analyses of normalized c EGFR and d γ-H2AX expressions in OVCAR-5 cells. e Mitochondrial membrane depolarization was assessed at 24 h post-light irradiation (0.35 J/cm2, 10 mW/cm2). (n = 3; *P < 0.05; **P < 0.01; ***P < 0.001; n.s.: not significant; one-way ANOVA, Tukey’s post hoc test)

Back to article page