Fig. 1

Isolation and molecular characterization of engineered exosomes. a Schematics of DNA constructs used for the production of fusion proteins LG (upper) and THLG (lower). b Fluorescence images of the GFP, LG and THLG in 293T cells ( ×400). c Biomarkers of exosomes were determined by western blot analysis of exosomes. d Western blot analysis of GFP in exosomes obtained from supernatants of GFP-293T cells, LG-293T cells and THLG-293T cells. e Confocal microscopy of exosomes isolated from 0.22 filtered conditioned medium of GFP-293T cells, LG-293T cells and THLG-293T cells ( ×1000). f Transmission electron micrograph of THLG-EXO and THLG-EXO/5-FU/miR-21i. g Size distribution of THLG-EXO and THLG-EXO/5-FU/miR-21i measured by DLS