Skip to main content
Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Nanobody‑horseradish peroxidase and -EGFP fusions as reagents to detect porcine parvovirus in the immunoassays

Fig. 2

Construction of the VHH library and screening the PPV-VP2 specific nanobodies by phage display technology. a Titres of antibodies against PPV-VP2 protein in the sera from the Camel after the fifth immunisation. b 48 clones were randomly picked to estimate the correct insertion rate by PCR. The size of PCR products was approximately 700 bp. c Identification of the periplasmic extracts from the 96 clones specifically binding to the PPV-VP2 protein with indirect ELISA. 90 clones were identified as positive. d Alignment of the amino acid sequences of 5 screened nanobodies against PPV-VP2 protein. The sequences are grouped according to their CDRs. e Specific reactions between the 5 screened nanobodies and PPV-VP2 protein

Back to article page

Journal of Nanobiotechnology

ISSN: 1477-3155

Contact us