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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Nanobody‑horseradish peroxidase and -EGFP fusions as reagents to detect porcine parvovirus in the immunoassays

Fig. 3

Expression, purification and identification of the 5 recombinant nanobodies against PPV-VP2 protein by prokaryotic system expression. a SDS-PAGE analysis of expression and purification of the 5 PPV-VP2 specific nanobodies by E. coli. M: Marker; lanes 1, 5, 9, 13, 17: Un-induced of PPV-VP2-Nb5, -Nb12, -Nb18, -Nb19 and -Nb56, respectively; lanes 2, 6, 10, 14, 18: Supernatant of PPV-VP2-Nb5, -Nb12, -Nb18, -Nb19 and -Nb56 after sonication, respectively; lanes 3, 7, 11, 15, 19: Precipitation of PPV-VP2-Nb5, -Nb12, -Nb18, -Nb19 and -Nb56, respectively; lanes 4, 8, 12, 16, 20: Purification of PPV-VP2-Nb5, -Nb12, -Nb18, -Nb19 and -Nb56, respectively. b Detection of the 5 recombinant nanobodies specifically binding to the PPV-VP2 protein with the indirect ELISA

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Journal of Nanobiotechnology

ISSN: 1477-3155

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