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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Nanobody‑horseradish peroxidase and -EGFP fusions as reagents to detect porcine parvovirus in the immunoassays

Fig. 4

Expression and characterisation of the 5 PPV-VP2-Nbs-HRP fusions in the HEK293T cells. a Identification of PPV-VP2-Nbs-HRP expressed in the HEK293T cells by IFA. b Detection of the HRP activity in the PPV-VP2-Nbs-HRP fusions secreted into the culture medium of HEK293T cells. c Titres of the PPV-VP2-Nbs-HRP in the medium reaction with the PPV-VP2 protein by direct ELISA. The blank vector (Empty-HRP) was as the negative control. d Detection of the PPV in the ST cells with PPV-VP2-Nbs-HRP fusions as the first antibody by IFA. PPV positive swine serum as the positive control; PPV negative swine serum and supernatant of HEK293T cells transfected with blank vector as the negative control

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Journal of Nanobiotechnology

ISSN: 1477-3155

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