Fig. 5From: Nanobody‑horseradish peroxidase and -EGFP fusions as reagents to detect porcine parvovirus in the immunoassaysExpression and characterisation of the 5 nanobody-EGFP fusions in the HEK293T cells. a Schematic presentation of the commercial vector pEGFP-N1 changed into the vector to insert the main genes encoding IgG signal peptide and multiple cloning site (MCS). b Detection of the expression of PPV-VP2-Nbs-EGFP in the HEK293T cells with direct fluorescence assay. c Detection of the PPV-VP2-Nbs-EGFP secreted into the culture medium of HEK293T cells by Western blot. Lanes 1-6: PPV-VP2-Nb5-EGFP, PPV-VP2-Nb12-EGFP, PPV-VP2-Nb18-EGFP PPV-VP2-Nb19-EGFP, PPV-VP2-Nb56-EGFP and Empty-EGFP (blank vector as negative control), respectively. d Titres of the PPV-VP2-Nbs-EGFP in the medium reacting with the PPV-VP2 protein by indirect ELISA. e Detection of PPV in the ST cells with the 5 PPV-VP2-Nbs-EGFP fusions by direct fluorescent assayBack to article page