Table 3 Optimised amount of PPV-VP2-Nb19 as the capture antibody and dilution of PPV-VP2-Nb56-HRP fusions in the medium as the detection antibody using the developed sandwich ELISA-like assay
Different amounts of PPV-VP2-Nb19 (ng/well) | Samples | Dilutions of the PPV-VP2-Nb56-HRP fusions as detection antibody | ||||
|---|---|---|---|---|---|---|
1:1 | 1:10 | 1:100 | 1:1000 | 1:10,000 | ||
8000 | Positive | 0.88 | 0.92 | 0.96 | 0.61 | 0.42 |
Negative | 0.06 | 0.05 | 0.05 | 0.06 | 0.06 | |
P/N | 14.67 | 18.40 | 19.2 | 10.17 | 7.00 | |
4000 | Positive | 0.75 | 0.80 | 1.04 | 0.50 | 0.34 |
Negative | 0.06 | 0.05 | 0.05 | 0.06 | 0.05 | |
P/N | 12.50 | 16.00 | 20.80 | 8.33 | 6.8 | |
2000 | Positive | 0.73 | 0.77 | 0.87 | 0.46 | 0.30 |
Negative | 0.05 | 0.06 | 0.05 | 0.05 | 0.04 | |
P/N | 14.6 | 12.83 | 17.4 | 9.20 | 7.5 | |
1000 | Positive | 0.74 | 0.72 | 0.82 | 0.43 | 0.25 |
Negative | 0.05 | 0.04 | 0.05 | 0.06 | 0.06 | |
P/N | 14.8 | 18.00 | 16.40 | 7.17 | 4.17 | |