Fig. 1

Incorporation of LNCs of different size by peripheral blood leukocytes (PBLs). a PBLs from 3 HDs were treated for 90′ with neutral DiD-LNCs of different size (25 nm in black, 50 nm in orange and 100 nm in blue), with DiD at 50 ng/ml and then stained with mAbs (anti-CD3, anti-CD14, anti-CD19, anti-CD56, anti-CD11b, anti-CD16) for flow cytometry analysis. Blank-LNCs were used as negative control. b PBLs from 3 HDs were treated for 3 h with 50 nm and 100 nm DiD-LNCs at a DiD concentration of 50 ng/ml and stained with anti-CD14 and anti-CD3 mAbs to identify monocyte (black) and T lymphocyte (grey) uptake. c The histogram in panel B shows the ratio between the percentage of DiD⁺ cells among CD14⁺ and CD3⁺ populations. Mean and standard error (SE) of 3 independent experiments are reported. Student t-test was performed, *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001