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Fig. 16 | Journal of Nanobiotechnology

Fig. 16

From: Iron oxides nanoparticles (IOs) exposed to magnetic field promote expression of osteogenic markers in osteoblasts through integrin alpha-3 (INTa-3) activation, inhibits osteoclasts activity and exerts anti-inflammatory action

Fig. 16

IOs and MF induce apoptosis in osteoclasts. Expression of apoptosis related genes including p21 (a), p53 (b), CASP-9 (c), BAX (d) and BCL-2 (e). Furthermore, based on the relative gene expression the ratio of BAX:BCL-2 was calculated (f). Representative graphs from Muse® Annexin V and Dead Cell Assay Kit (g). Based on the obtained data, percentage of live cells (h), early apoptotic cells (i), late apoptotic/dead cells (j) and dead cells (k) was calculated. Results expressed as mean ± SD. Statistical significance indicated as asterisk (*) when comparing the results between corresponding bars representing MF− and MF+ groups, and as number sign (#) when comparing to CTRL MF−. #,*p < 0.05, ##, **p < 0.01, ***, ###p < 0.001

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