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Fig. 17 | Journal of Nanobiotechnology

Fig. 17

From: Iron oxides nanoparticles (IOs) exposed to magnetic field promote expression of osteogenic markers in osteoblasts through integrin alpha-3 (INTa-3) activation, inhibits osteoclasts activity and exerts anti-inflammatory action

Fig. 17

IOs and MF affect mitochondrial membrane potential and contributes to enhance apoptosis in osteoclasts. Representative photographs from Calcein A.M. and Propidium iodide (A) and their quantification (B) revealed increased number of apoptotic cells in IOs and MF groups. Western blot analysis revealed increased amount of CASP-3 in IOs MF− and IOs MF+ groups. To investigate mitochondrial condition in cells Muse® MitoPotential analysis was performed (D). Based on the obtained data, percentage of live cells (E), depolarized/live cells (F), depolarized/dead cells (G) and dead cells (H) was calculated. Results expressed as mean ± SD. Statistical significance indicated as asterisk (*) when comparing the results between corresponding bars representing MF− and MF+ groups, and as number sign (#) when comparing to CTRL MF−. #,*p < 0.05, ##, **p < 0.01, ***, ###p < 0.001

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