Fig. 6

Target identification by using GNP-6. G. kobayashii cell lysate (300 µL) was incubated with (lane 3) or without (lane 4) 5 mM ARG for 1 h at 4 °C, after which 0.06 μmol of GNP-6 was added and the mixture incubated for 1 h at 4 °C. As a control, parasite extract (300 μL) was also incubated with GNP-10 and treated in the same way (lane 2). Proteins bound to GNPs were separated by 12.5% SDS-PAGE followed by improved Coomassie brilliant blue G-250 staining. Protein bands with lower intensities in the lane with ARG than in the lane without ARG were identified by MALDI-TOF/TOF MS and Mascot analysis (Table 1)