Fig. 5

Comparative analyses of NEs from the wild type and tt mutants. a Each of the heatmap columns represents the normalized intensity averaged across the NE samples within each group (n = 3, each sample was a pool of tissues from separate plants). Hierarchical clustering analysis was performed on metabolites with significant factor loadings in PC1 (p = 0.005) using Manhattan correlation as the distance measure. Percentage of lipids (green), phenylpropanoids (PPP; purple) and other compounds (gray) that form each cluster is noted on the right-hand side. The color intensity scale is positioned below the heatmap. b Boxplot analyses of ME levels of select lipids that have been enriched in the NEs of tt4. The significance between means (n = 3) was calculated using Students t-test. **p < 0.01