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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Manipulation of macrophage polarization by peptide-coated gold nanoparticles and its protective effects on acute lung injury

Fig. 3

The preferential uptake of P12 by pulmonary macrophages upon intratracheal administration. Liu staining of cells in the BALF collected from three experimental groups: a PBS + PBS, b PBS + LPS, and c P12 + LPS group; the red arrows indicate pulmonary macrophages with internalized P12 in dark blue color; scale bar represents 60 µm. d Gating strategy of flow cytometry analysis to identify monocytes (Mo), dendritic cells (DCs), macrophages (Mφ), neutrophils, T cells and B cells in the BALF. e The amount of internalized P12 in various immune cells in the BALF using the mean fluorescence intensity (MFI) of Cy5-labeled P12 (P12-Cy5); N = 4, ***p < 0.001. f Uptake of P12-Cy5 nanoparticles in mononuclear phagocytic immune cells: macrophages (left), DCs (middle) and monocytes (right); the P12-Cy5 treated group shown in blue and the corresponding untreated group shown in red

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