Fig. 3From: Manipulation of macrophage polarization by peptide-coated gold nanoparticles and its protective effects on acute lung injuryThe preferential uptake of P12 by pulmonary macrophages upon intratracheal administration. Liu staining of cells in the BALF collected from three experimental groups: a PBS + PBS, b PBS + LPS, and c P12 + LPS group; the red arrows indicate pulmonary macrophages with internalized P12 in dark blue color; scale bar represents 60 µm. d Gating strategy of flow cytometry analysis to identify monocytes (Mo), dendritic cells (DCs), macrophages (Mφ), neutrophils, T cells and B cells in the BALF. e The amount of internalized P12 in various immune cells in the BALF using the mean fluorescence intensity (MFI) of Cy5-labeled P12 (P12-Cy5); N = 4, ***p < 0.001. f Uptake of P12-Cy5 nanoparticles in mononuclear phagocytic immune cells: macrophages (left), DCs (middle) and monocytes (right); the P12-Cy5 treated group shown in blue and the corresponding untreated group shown in redBack to article page