Fig. 6

The effects of P12 on the polarization of BMDMs to M2 phenotype. Under IL-4 and IL-13 co-stimulation for M2 polarization, cytokine levels of IL-12/23p40 (a), IL-12p70 (b), IL-6 (c) and IL-10 (d) in the macrophage culture supernatants were quantified by ELISA 24 h after stimulation in the presence or absence of P12 treatment. The mRNA levels of M1 markers of iNOS (e) and IL-12 (f), and the M2 markers of ARG1 (g) and YM1 (h) were analyzed by qRT-PCR. P12 concentration = 12.5 and 50 nM, N = 6, *p < 0.05, **p < 0.01