Fig. 7

The effects of P12 on pulmonary macrophage polarization in the LPS-induced ALI mouse model. a Gating strategy of flow cytometry analysis to identify the M1 and M2 phenotypes of interstitial macrophages (IMs) and tissue-resident alveolar macrophages (AMs) in the BALF and single cell suspensions of lung tissues. Percentages of M1 macrophages in AM (b, f) and IM (c, g) populations, and M2 macrophages in AM (d, h) and IM (e, i) in the BALF (b–e) as well as in the lung (f–i) upon P12 treatment compared to the LPS group. N ≥ 11, ns: not significant, ****p < 0.0001