Fig. 2

Production and characterization of the 5 fenobodies against NDV particles. a SDS-PAGE analysis of the 5 fenobodies against NDV with bacterial system for expression and with Ni–NTA-6FF Column for purification. Lanes 1–5: NDV-fenobody-2, -4, -24, -30 and -49. b Western blot analysis of the purified 5 fenobodies against NDV reacting with anti-His monoclonal antibody. Lanes 1–5: NDV-fenobody-2, -4, -24, -30 and -49. c TEM analysis of the 5 purified fenobodies self-assembled into the 24 subunit nanocage. A picture of the NDV-fenobody-4 is shown here, which is same as 4 other fenobodies. d Analysis of the 5 fenobodies specifically binding with the NDV particles with indirect ELISA and the anti-H9N2 fenobody as the negative control